Education - Transcriptome Analysis Workshop

Transcriptome Analysis Workshop

This workshop will provide a detailed introduction to transcriptome sequencing and informatics concepts. It will provide training on analyses of RNA-Seq data including the use of different analytical tools, relevant file formats, reference genomes, transcriptome annotations, quality control measures, reference-based alignment, gene expression, and differential gene and isoform analyses. These tutorials will be accompanied by hands-on exercises using complete analytical pipelines on test datasets.

Included topics

  • Transcriptome sequencing techniques
  • QC considerations
  • Read alignment with Bowtie, and HISat
  • Expression quantification using HTSeq
  • Differential expression with DEseq
  • Isoform analysis with Cufflinks
  • Differential Isoform analysis with CuffDiff
  • Pathway Analysis with IPA, GOrilla, and DAVID
  • Primer on single-cell RNASeq

Dates and Registration

The registration fee for this workshop is $450 for general rate, $350 for student rate. This includes breakfast and lunch for the two full days of the workshop.

The workshop has concluded, please check back for future dates

Location and Times

The course will be held on the University of Maryland Baltimore campus in the Health Sciences Facility, Building 3, at 670 W. Baltimore St., Baltimore, MD 21201 [Google map].

These workshops are 2.5 days long. They will begin in the afternoon of the first day with registration/check-in from 12:30 to 1:00. The workshop will run from 1:00-5:00 the first day and from 9:00-5:00 the remaining two days. Breakfast and lunch will be provided on the second and third days (the two full days).

Driving Directions

Directions can be found here. Parking is available for $9.00 per day in a garage at the corner of W. Baltimore and Poppleton, this is about 2 blocks from our building. Street parking is available.


The closest airport is Baltimore Washington International Thurgood Marshall Airport (BWI). Other airports in the region (1 - 1.5 hours away) are Dulles and Reagan National.


Baltimore is served by Amtrak and MARC train service.


There are several hotels within easy walking distance to our location at 670 W. Baltimore St. Here are a couple of suggestions that previous attendees have been happy with:

Hampton Inn Baltimore-Downtown-Convention Center
Holiday Inn Baltimore-Inner Harbor (Downtown)


Please send any questions to Michelle Giglio (mgiglio AT